Article Title:Optogenetic protein clustering through fluorescent protein tagging and extension of CRY2 Article Snippet:..Eion plasmids for pCypet-C1 (Addgene plasmid #54649, a gift from Patrick Daugherty and Michael Davidson), pYpet-C1 (Addgene plasmid #54648, a gift from Patrick Daugherty and Michael Davidson), FusionRed-C1 (Addgene plasmid #54777, a gift from Michael Davidson) ,
Septin 2/6/7 complexes tune microtubule plus-end growth SEPT2/6/7 tunes MT plus-end dynamics in a concentration-dependent manner. Using a TIRF microscopy assay that visualizes the polymerization and depolymerization of Rhodamine-labeled tubulin onto stable HiLyte 488-labeled MT seeds, we tested how MT dynamics are impacted by different concentrations of recombinant SEPT2/6/7 complexes, which were purified from bacteria.
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pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA Addgene Saunders A, Johnson C, Sabatini B. Novel recombinant adeno-associated viruses for Cre activated and inactivated transgene eion in neurons.
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